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Evaluation of operational errors and statistical analysis of replicate determinations of iron in water using a micro plate.
This discusses the use of UV-Visable spectrophotemtry and the use of assays involving a microplate. statistical analysis is also then given of the data gained.
|language || ||english
|wordcount || ||2217 (cca 6 pages)
|contextual quality || ||N/A
|language level || ||N/A
|price || ||free
|sources || ||1
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Preview of the essay: Evaluation of operational errors and statistical analysis of replicate determinations of iron in water using a micro plate.
UV-Visible Spectrophotometry Evaluation of operational errors and statistical analysis of replicate determinations of iron in water using a micro plate. Introduction. Large sets of data can be analysed using the overall variance, to determine errors in the preparation of the analytical sample. The uses of large replicate samples also allow errors in precision to be identified. To begin with five identical samples and a blank were prepared in 25cm3 volumetric flasks, using a bulb piptte. In this part of experiment errors that arise are from pipetting, mixing and colour development. Using a UV-VIS spectrophotometer, the absorbance of the blank and sample were taken, in ...
... of the microtitre plate; fingerprints on the base of the microtitre plate would produce inaccurate absorbance readings. From the coefficient of variance values, it is obvious that solution 3 is the most precise as the values are close to one another and having a value 0.50%. However solution 4 encountered the most errors with a value of 2.58%. This laboratory procedure analysis precision, however to assess the accuracy of these procedures, you can asses the absorbance reading of the plate reader to known values, compare the values.
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