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Determination of Molecular mass of a Protein and investigation of Homogeneity
This is an experimental write up which looks into the use of SDS-page assays to determine molecular mass of proteins.
|language || ||english
|wordcount || ||1509 (cca 4 pages)
|contextual quality || ||N/A
|language level || ||N/A
|price || ||free
|sources || ||2
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Preview of the essay: Determination of Molecular mass of a Protein and investigation of Homogeneity
Protein Electrophoresis SDS-PAGE Determination of molecular mass of a protein and investigation of homogeneity. Introduction Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a technique used to separate proteins according to their molecular mass. SDS is a detergent used to denature proteins, and apply a negative charge identical to the proteins mass. Due to proteins being colourless, Bromophenol Blue a tracking dye is used during electrophoresis, to keep ...
... marker protein. This is because the volume of the unknown protein was 17 µl, which is larger, compared to that of the 8 µl marker protein.
From the gel obtained, the homogeneity of the ‘unknown’ protein sample can be determined as being very poor. This is apparent from the 8 contaminants visible, which shows that the protein composition throughout was not homogeneous.
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